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Conducting scaffolds for liver tissue engineering

Significance Statement

The high proliferation capacity of stem cells, cancer cells and even proliferated cells, can be attributed to their specific cell membrane potential; the more positive membrane voltage, the higher the proliferation rate. For example, liver cell is recognized as one of the most depolarized of all differentiated cells, while liver is known as one of the organs with highest regeneration capability. The phenomenon can be emphasized by growing cells in a scaffold that contains specific protein parts to enhance chemical signaling, and conducting parts to improve electrical signaling among cells. The conducting part can regulate and adjust the membrane voltage in a way to improve the proliferation and regeneration rate of the body. This point has been investigated in this paper and it was shown that conductivity of the scaffolds increases the proliferation of hepatocyte cells.

Conducting scaffolds for liver tissue engineering

Journal Reference

Journal of Biomedical Materials Research, Part A DOI: 10.1002/jbm.a.35080. (2014).

Armin Tahmasbi Rad, Naushad Ali, Hari Shankar R. Kotturi, Mostafa Yazdimamaghani, Jim Smay, Daryoosh Vashaee, Lobat Tayebi.

 

School of Materials Science and Engineering, Helmerich Advanced Technology Research Center, Oklahoma State University, Tulsa, Oklahoma 74105 and

Department of Internal Medicine, Section of Digestive Diseases and Nutrition, University of Oklahoma Health Sciences Center, 975 NE 10th Street, Oklahoma City, Oklahoma 73104 and

Department of Biology, University of Central Oklahoma, 100 North University Drive, Edmond, Oklahoma 73034 and

School of Chemical Engineering, Oklahoma State University, Stillwater, Oklahoma 74078 and

School of Electrical and Computer Engineering, Helmerich Advanced Technology Research Center, Oklahoma State University, Tulsa, Oklahoma 74105.

 Abstract

 It is known that there is a correlation between a cell membrane potential and the proliferation of the cell. The high proliferation capacity of liver cells can also be attributed to its specific cell membrane potential as liver cell is recognized as the most depolarized of all differentiated cells. We hypothesized that this phenomenon can be emphasized by growing liver cells in conductive scaffolds that can increase the electrical communication among the cells. In this paper, using tissue engineering techniques, we grew hepatocyte cells in scaffolds with various compositions. It was found that the scaffolds containing conducting polymer of Poly (3, 4-ethylenedioxythiophene) (PEDOT) provide the best condition for attachment and proliferation of the cells. More specifically, the blend of Hyaluronan, PEDOT and Collagen (I) as dopants in Gelatin-Chitosan based scaffold presented the best cell/scaffold interactions for regeneration of liver cells.

 

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