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Testing the Concept of the Interatomic Status of the NFE2L2/AP1 Pathway as a Systemic Biomarker for Examination Stress.

Zolotukhin PV1, Dovzhik AD, Lebedeva UA, Kuzminova ON, Mashkina EV, Aleksandrova AA, Shkurat TP.

Mol Diagn Ther. 2014 Feb 7th.

Laboratory of Biomedicine, Research Institute of Biology, Southern Federal University, Stachki av., 194/1, 344090, Rostov-on-Don, Russia, [email protected]

Abstract

 

BACKGROUND AND OBJECTIVES:

Oxidative status-based interactomic profiling is a promising approach for fundamental integrative cell biology, diagnostics, and therapy. However, this approach has been neither utilized as a method nor tested as a tool. Thus, we aimed (1) to develop an oxidative status pathway state assessment-based analytical procedure relying on NFE2L2/AP1 pathway evaluation, and (2) to preliminarily assess its responsiveness, performance and diagnostic properties when applied to deciphering stress conditions of the academic examination period and academic term. These conditions were chosen as those representing a common model of mild, everyday-life stressors causing shifts in oxidative status.

METHODS:

To meet the aim of the study, we performed a repetitive-measurements study collating gene expression of NFE2L2/AP1pathway targets and controllers under the two stress conditions using semi-quantitative reverse transcription-polymerase chain reaction.

RESULTS:

Surprisingly, even with some sensitivity limitations of the methods employed, a pathway state analysis approach based on a multiple target-to-controller ratio calculation was highly responsive and yielded very high receiver operating characteristics in deciphering the model stress conditions.

CONCLUSION:

Although further testing of the approach is required, the interactomic pathway activation assaying concept was preliminarily experimentally proven to be a highly promising clinical diagnostic tool that may easily be adapted for current tasks.

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Additional Information

Assessing pathways’ states using interactomic data is a powerful approach for collecting information on the signaling background and adaptive performance of cellular systems. In the present paper we demonstrate the outlooks for applied oxidative status-based interactomics in the field of molecular medicine. To advance the technology, we would like to emphasize the need for further deep investigations of pathways functioning fundamentals – from pathway interference events and to individual control of expression of transcript variants.

 

Testing the Concept of the Interatomiay as a Systemic Biom