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Synthesis and characterization of hyaluronic acid/human-like collagen hydrogels

Zhang J1, Ma X2, Fan D3, Zhu C1, Deng J1, Hui J1, Ma P1. Mater Sci Eng C Mater Biol Appl. 2014 Oct 1;43:547-54.

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1Shaanxi Key Laboratory of Degradable Biomedical Materials, Department of Chemical Engineering, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi’an, Shaanxi 710069, China.

2Shaanxi Key Laboratory of Degradable Biomedical Materials, Department of Chemical Engineering, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi’an, Shaanxi 710069, China. Electronic address: [email protected]

3Shaanxi Key Laboratory of Degradable Biomedical Materials, Department of Chemical Engineering, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi’an, Shaanxi 710069, China. Electronic address: [email protected]

 

Abstract

Injectable hydrogel plays an important role in soft tissue filling and repair. We report an injectable hydrogel based on hyaluronic acid (HA) and human-like collagen (HLC), both with favorable biocompatibility and biodegradability. These two types of biomacromolecules were crosslinked with 1,4-butanediol diglycidyl ether to form a three-dimensional network. The redundant crosslinker was removed by dialysis and distillation. An HA-based hydrogel prepared by the same method was used as a control. The cytocompatibility was studied with a Cell Counting Kit-8 (CCK-8) test. Carbazole colorimetry was used to analyze the in vitro degradation rate. The histocompatibility was evaluated by hematoxylin and eosin (H&E) staining analysis and immunohistochemical analysis. The CCK-8 assay demonstrated that the HA/HLC hydrogel was less cytotoxic than the HA-based hydrogel and could promote baby hamster kidney cell (BHK) proliferation. The cell adhesion indicated that BHK could grow well on the surface of the materials and maintain good cell viability. The in vitro degradation test showed that the HA/HLC hydrogel had a longer degradation time and an excellent antienzyme ability. In vivo injection showed that there was little inflammatory response to HA/HLC after 1, 2, and 4 weeks. Therefore, the HA/HLC hydrogel is a promising biomaterial for soft tissue filling and repair.

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