Home » Key Scientific Articles » Redox control of indoleamine 2,3-dioxygenase expression and activity in human monocyte-derived dendritic cells is independent of changes in oxygen tension

Redox control of indoleamine 2,3-dioxygenase expression and activity in human monocyte-derived dendritic cells is independent of changes in oxygen tension

Mattox ML, D’Angelo JA, Dickinson BL.

Scand J Immunol. 2014 May;79(5):325-32.

The West Virginia School of Osteopathic Medicine, Lewisburg, WV, USA.

 

Abstract

Dendritic cells (DCs) initiate adaptive immune responses to pathogens and tumours and maintain tolerance to self and innocuous antigens. These functions occur in organs and tissues exhibiting wide variations in nutrients, growth factors, redox and oxygen tension. Understanding how these microenvironmental factors influence Dendritic cells to affect immunological outcomes is of increasing relevance with the emerging success of Dendritic cell-based cellular vaccines. In a previous study, we examined whether redox, an important environmental cue, could influence Dendritic cell expression of the immunosuppressive enzyme indoleamine 2,3-dioxygenase (IDO). indoleamine 2,3-dioxygenase-competent Dendritic cells promote long-term immune homoeostasis by limiting exaggerated inflammatory responses and directing regulatory T-cell effector function. To alter redox, we manipulated the activity of the cystine/glutamate antiporter, which functions to maintain intracellular and extracellular redox. The results of that study showed that redox perturbation strongly induced indoleamine 2,3-dioxygenase expression and activity in Dendritic cells. While this study was performed using standard cell culture techniques with Dendritic cells cultured under 5% CO₂ and 20% O₂, it is clear that Dendritic cells capture and present antigens in inflamed tissues and secondary lymphoid organs which exhibit low oxygentension (1-5% O₂). Therefore, here we investigated whether oxygen tension influences Dendritic cells expression of indoleamine 2,3-dioxygenase in the context of homoeostatic and altered redox.

 

© 2014 John Wiley & Sons Ltd.

Go To PubMed

 

 

Redox control of indoleamine 2,3-dioxygenase expression and activity in human monocyte-derived dendritic cells - Global Medical Discovery