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Ionized gas (plasma) delivery of reactive oxygen species (ROS) into artificial cells

Journal of Physics D: Applied Physics Volume 47 Number 36, 2014.

Sung-Ha Hong1,4, Endre J Szili2,3,4, A Toby A Jenkins1 and Robert D Short2,3

1 Department of Chemistry, University of Bath, Bath, BA2 7AY, UK and
2 Mawson Institute, University of South Australia, Adelaide, SA 5095, Australia and
3 Authors to whom any correspondence should be addressed. and
4 These authors are co-first authors.

 

Abstract 

This study was designed to enhance our understanding of how reactive oxygen species (ROS), generated ex situ by ionized gas (plasma), can affect the regulation of signalling processes within cells. A model system, comprising of a suspension of phospholipid vesicles (cell mimics) encapsulating a ROS reporter, was developed to study the plasma delivery of ROS into cells. For the first time it was shown that plasma unequivocally delivers ROS into cells over a sustained period and without compromising cell membrane integrity. An important consideration in cell and biological assays is the presence of serum, which significantly reduced the transfer efficiency of ROS into the vesicles. These results are key to understanding how plasma treatments can be tailored for specific medical or biotechnology applications. Further, the phospholipid vesicle ROS reporter system may find use in other studies involving the application of free radicals in biology and medicine

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note: Corrections to the original manuscript can be found in Hong et al JPhysD 47 (2015) 029501.