Home » Key Scientific Articles » The human natural killer cytotoxic cell line NK-92, once armed with a murine CD16 receptor, represents aconvenient cellular tool for the screening of mouse mAbs according to their ADCC potential.

The human natural killer cytotoxic cell line NK-92, once armed with a murine CD16 receptor, represents aconvenient cellular tool for the screening of mouse mAbs according to their ADCC potential.

Clémenceau B, Vivien R, Pellat C, Foss M, Thibault G, Vié H.

MAbs. 2013 Jul-Aug;5(4):587-94.

INSERM U892, Nantes, France. [email protected]

 

Abstract

 

To take advantage of the large number of well-characterized mouse immunoglobulins (IgGs) for the study of antibody-dependent cell-mediated cytotoxicity (ADCC) in human cells, we armed human cytotoxic lymphocytes with a mouse receptor for the Fc portion of IgG antibodies. The humanΝΚ-92 natural killer cell line was transduced with a mouse receptor gene (mCD16), which was stably expressed on the cell surface (referred to as NK-92 (mCD16) ). When tested against a B-lymphoblastoid cell line (BLCL) coated with mouse anti-CD20 IgG1, IgG2a or IgG2b monoclonal antibodies (mAbs), the newly expressed mouse Fc receptor enabled the NK-92 (mCD16) cells to kill the BLCL by ADCC. Next, using the NK-92 (mCD16) we compared mouse mAbs directed at B lineage specific CD antigens for their ability to induce ADCC against human Epstein-Barr virus- infected B lymphoblastoid (for anti-CD19, -CD20 and -CD21) or against myeloma (for anti-CD38 and -CD138) target cells. Our results demonstrated that the “NK-92 (mCD16) assay” allows convenient and sensitive discrimination of mouse mAbs for their ability to mediate ADCC in a human cellular system. In addition, our results provide examples of dissociation between opsonization and target cell killing through ADCC. These “murinized” human effector cells thus represent a convenient cellular tool for the study of ADCC.

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