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Effect of chemical composition on the response of zwitterionic glucose sensitive hydrogels studied by design of experiments

Significance statement

Co-polymer hydrogels containing pendant phenylboronic acid functional groups are synthetic lectins that can be designed to specifically bind glycosylated proteins containing cis-diols.  Stimuli-responsive hydrogels are crosslinked polymer networks that have swelling degrees that autonomously respond to changes in the environmental concentration of analytes such as glucose. In this work, we employ a design-of-experiments approach to obtain stimuli-responsive hydrogels that selectively respond to glucose concentration changes in the environment with fast response times.  A similar approach can be used to obtain stimuli-responsive hydrogels that respond to glucose concentration changes within the cell culture media present in bioreactors, with use of noninvasive magnetometers to transduce the hydrogel swelling response into wireless signals for bioprocess control.

Journal Reference

Seung-Hei Cho1, Prashant Tathireddy2,Loren Rieth2 , Jules Magda1,* J. Appl. Polym. Sci. 2014, 131, 40667.

  1. Department of Chemical Engineering, University of Utah, Salt Lake City, Utah and
  1. Department of Electrical and Computer Engineering, University of Utah, Salt Lake City, Utah.

Abstract

A stimuli-responsive hydrogel that contains the anionic monomer 3-acrylamidophenylboronic acid and the cationic monomer N-[3-(dimethylamino)propyl]acrylamide binds with cis-diol groups of glucose molecules selectively and reversibly. Even though such hydrogels have good selectivity for glucose, there are still remaining thresholds that should be overcome to enhance the sensitivity (swelling pressure response magnitude) and to reduce the response time (inverse of 1st order rate constant). In this study, the sensitivity and response time of zwitterionic glucose sensitive hydrogels (GSHs) were studied with three factor DOE analysis. The DOE results show that the molar ratio of 3-acrylamidophenylboronic acid/N-[3-(dimethylamino)propyl]acrylamide and the wt % of monomer in the pregel solution are the most important factors for enhancing the hydrogel sensitivity. In addition, fast response times can best be achieved by decreasing the molar ratio of cross-linker. The results of this study will be useful as guidelines for the optimal synthesis of glucose sensitive hydrogels.

Copyright © 2014 Wiley Periodicals, Inc.

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