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Nanoparticle-Dna-Polymer Composites For Hepatocellular Carcinoma Cell Labeling, Sensing, And Magnetic Resonance imaging

Significance Statement

The HepG2 cell transfection deficiencies involving NP uptake and gene expression with composites could be perturbed by tuning the ratio between PEI and NP (bare-, alginate-, and citrate-coated) components in eighteen composites, which have been characterized by Prussian blue staining, in vitro MRI, GPF fluorescence, and luciferase expression. Among the eighteen composites prepared, comparatively, 0.2 ng PEI/DNA/1.0 mg bare-NP composite possesses the best cellular uptake efficacy of NP to the cytoplasm while 0.2 ng PEI/DNA/0.1 µg alginate-NP possesses the best cellular uptake efficacy of the pDNA to the nucleus. That is, the sensing property in the receptor-mediated endocytosis between the composite and HepG2 cell mainly depends on the nature of the NP’s surface coating. Magnetofection has been found to enhance the cellular uptake efficacy and efficiency with the 0.1 ng PEI/DNA/1.0 µg alginate-NP composite by two  orders of magnitude compared to the cellular uptake with the composite but without an externally applied magnetic field, characterizing by their luciferase activity (RLU). The cell viabilities of HepG2 and MDCK vary from 70% to 90% in the presence of different composites.

 

Nanoparticle-DNA-polymer composites for hepatocellular carcinoma cell labeling

Journal Reference

Leung KCF, Lee SF, Wong CH,Chak CP, Lai JMY, Zhu XM, Wang YXJ, Sham KWY, Cheng CHK.

Methods. 2013 Dec 15:64(3):315-21.

Department of Chemistry, Institute of Creativity,Institute of Molecular Functional Materials, The Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong SAR, P. R. China and
Department of Chemistry, School of Biomedical Sciences, Department of Imaging and Interventional Radiology, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR, P. R. China

 

 

Abstract

This paper describes comparative studies and protocols in (1) self-assembling of ultrasmall superparamagnetic iron oxide nanoparticle (NP), circular plasmid DNA, and branched polyethylenimine (PEI) composites; (2) magnetofection; (3) gene delivery, (4) magnetic resonance imaging (MRI), and (5) cytotoxicity of the composites toward hepatocellular carcinoma HepG2 cells.

 

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