Home » Key Drug Discovery Articles » Inhibitory effects of the leaves of loquat (Eriobotrya japonica) on bone mineral density loss in ovariectomized mice and osteoclast differentiation.

Inhibitory effects of the leaves of loquat (Eriobotrya japonica) on bone mineral density loss in ovariectomized mice and osteoclast differentiation.

Significance Statement

Osteoporosis is a growing public health problem, affecting millions of people worldwide. We found one of natural resources, the leaves of Eriobotrya japonica could suppress the bone mineral density of ovariectomized mice and RANKL-induced osteoclast differentiation. The main active compound, ursolic acid was isolated through bio-guided isolation. We hope this finding could contribute new function of loquat leaves on prevention and treatment of osteoporosis.

 

Inhibitory effects of the leaves of loquat (Eriobotrya japonica) on bone mineral density loss in ovariectomized mice and osteoclast differentiation

Journal Reference

Tan H, Furuta S, Nagata T, Ohnuki K, Akasaka T, Shirouchi B, Sato M, Kondo R, Shimizu K.

J Agric Food Chem. 2014 Jan 29;62(4):836-41.

Department of Agro-environmental Sciences, Faculty of Agriculture, Kyushu University , 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.

Abstract

The loquat, Eriobotrya japonica Lindl. (Rosaceae), is a small tree native to Japan and China that is widely cultivated for its succulent fruit. Its leaves are used as an ingredient of a tasty tea called “Biwa cha” in Japanese. The anti-osteoporosis effects of the leaves of loquat in vitro and in vivo have been investigated. After 15 days of feeding normal diet or diet supplemented with 5% loquat leaves, the body weight, viscera weights, and bone mineral density (BMD) of both groups of eight ovariectomized (OVX) mice were compared. The result showed that the loss of BMD in loquat-fed micewas significantly prevented in three parts of the body, especially in the trabecular bone of the head (P < 0.05), abdomen (P < 0.01), and lumbar (P < 0.05) compared to the control group. No hypertrophy in the uterus by the loquat leaves diet was observed. The effect of the extract (447.25 g) prepared from the dried leaves of loquat (2.36 kg) was further studied on RANKL-induced osteoclast differentiation and cell viability. The extract suppressed the differentiation of osteoclasts under 50, 125, 250, and 500 ug/mL. Through bioactivity-guided fractionation, ursolic acid (1) was isolated and inhibited osteoclast differentiation under 4 and 10 ug/mL. It was concluded that loquat leaves possess the potential to suppress ovariectomy-induced bone mineral density deterioration.

 

Go To PubMed