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Expression of interleukin (IL)-19 and IL-24 in inflammatory bowel disease patients: a cross-sectional study.

Fonseca-Camarillo G, Furuzawa-Carballeda J, Granados J, Yamamoto-Furusho JK. Clin Exp Immunol. 2014;177(1):64-75.

Inflammatory Bowel Disease Clinic, Department of Gastroenterology, México City, México; Posgrado Ciencias Biológicas, Universidad Nacional Autónoma de México, México City, México.

 

Abstract

Interleukin (IL)-19 and IL-24 belong to the IL-20 subfamily, and are involved in host defence against bacteria and fungi, tissue remodelling and wound healing. Nevertheless, no previous studies have explored their expression in Mexican mestizo patients  with inflammatory  bowel disease (IBD). The aim of the study was to characterize and to enumerate peripheral and tissue IL-19- and IL-24-producing cells, as well as gene expression in patients with IBD with regard to its clinical activity. We studied a total of 77 patients with ulcerative colitis (UC), 36 Crohn’s disease (CD) and 33 patients as control group (without endoscopic evidence of intestinal inflammation). Gene expression was measured by real-time-polymerase chain reaction (RT-PCR). Protein expression was detected in biopsies by immunohistochemistry and in freshly isolated peripheral blood mononuclear cells by flow cytometry. IL-19 and IL-24 gene expression was elevated significantly in patients with active IBD versus the inactive disease and non-inflammatory control groups (P < 0·05). However, IL-19- and IL-24-producing cells were only increased in active CD versus active UC and non-inflammatory tissues (P < 0·05). IL-19 was produced conspicuously by circulating B cells and monocytes in patients with inactive disease (P < 0·05). Conversely, IL-24was noticeably synthesized by peripheral B cells, CD4(+) T cells, CD8(+) T cells and monocytes in patients with active disease. In conclusion, IL-19- and IL-24-producing cells in active CD patients were increased compared with active UC and non-inflammatory tissues. These cytokines could significantly shape and differentiate  inflammatory process, severity and tolerance loss between UC and CD pathophysiology.

© 2014 British Society for Immunology.

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Figure Legend

The interleukin (IL)-19 and IL-24 belong to the IL-20 subfamily, and are involved in host defense against bacteria and fungi, tissue remodeling and wound healing. Both cytokines were up-regulated in patients with IBD in the colonic mucosa and peripheral cells as a compensatory mechanism in order to decrease the inflammatory process as well as in the induction of tissue remodeling and mucosal healing through the production of mucins in the intestinal mucosa. These cytokines could be significantly shape and differentiate the inflammatory process, severity and tolerance loss between UC and CD pathophysiology.

 

Expression of interleukin (IL)-19 and IL-24 in inflammatory bowel disease patients: a cross-sectional study.. Global Medical Discovery