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Eliminating acute lymphoblastic leukemia cells from human testicular cell cultures: a pilot study.

Sadri-Ardekani H1, Homburg CH2, van Capel TM3, van den Berg H4, van der Veen F5, van der Schoot CE2, van Pelt AM6, Repping S5.

Fertil Steril. 2014 Apr;101(4):1072-1078.e1.

1Center for Reproductive Medicine, Women’s and Children’s Hospital, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands; Reproductive Biotechnology Research Center, Avicenna Research Institute, The Academic Center for Education, Culture and Research (ACECR), Tehran, Iran. Electronic address: [email protected] and

2Experimental Immunohematology, Sanquin Research at the Central Laboratory of the Netherlands Red Cross Blood Transfusion Service (CLB), Amsterdam, the Netherlands and

3Departments of Cell Biology and Histology, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands and

4Department of Pediatric Oncology, Women’s and Children’s Hospital, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands and

5Center for Reproductive Medicine, Women’s and Children’s Hospital, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands and

6Center for Reproductive Medicine, Women’s and Children’s Hospital, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands. Electronic address: [email protected]

 

Abstract

OBJECTIVE:

To study whether acute lymphoblastic leukemia (ALL) cells survive in a human testicular cell culture system.

DESIGN:

Experimental laboratory study.

SETTING:

Reproductive biology laboratory, academic medical center.

PATIENT(S):

Acute lymphoblastic leukemia cells from three patients and testicular cells from three other patients.

INTERVENTION(S):

Acute lymphoblastic leukemia cells were cultured alone or in combination with testicular cells, at various concentrations, in a system that has recently been developed to propagate human spermatogonial stem cells.

MAIN OUTCOME MEASURE(S):

Viability of ALL and testicular cells during culture was evaluated by flow cytometry using markers for live/dead cells. Furthermore, the presence of ALL cells among testicular cells was determined by highly sensitive (1:10,000 to 1:100,000 cells) patient-specific antigen-receptor minimal residual disease polymerase chain reaction. The presence of spermatogonia at the end of culture was determined by reverse transcription-polymerase chain reaction for ZBTB16, UCHL1, and GPR125.

RESULT(S):

The ALL cells cultured separately did not survive beyond 14 days of culture. When cultured together with testicular cells, even at extremely high initial concentrations (40% ALL cells), ALL cells were undetectable beyond 26 days of culture. Reverse transcription-polymerase chain reaction confirmed the presence of spermatogonia at the end of the culture period.

CONCLUSION(S):

Our pilot study shows that the described testicular cell culture system not only allows for efficient propagation of spermatogonial stem cells but also eliminates contaminating ALL cells.

Copyright © 2014 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

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N0009927 Photomicrograph; acute lymphocytic leukaemia